Journal: Glia

Article Title: Inhibition of inflammatory cells delays retinal degeneration in experimental retinal vein occlusion in mice

doi: 10.1002/glia.23739

Figure Lengend Snippet: Results from mouse cytokine antibody microarray for a selection of (a) proinflammatory and (b) anti‐inflammatory proteins. Significance levels: * p < .05, ** p < .01, *** p < .001, **** p < .0001; ♦: expression level below detection threshold (0.1 pg/ml)

Article Snippet: After washing and drying steps, a glass slide scanner with a Cy3 filter was used for fluorescence intensity detection (ScanRI Microarray Scanner, PerklinElmer, Inc., MA).

Techniques: Microarray, Selection, Expressing

Journal: International Journal of Environmental Research and Public Health

Article Title: Molecular Detection of a Potentially Toxic Diatom Species

doi: 10.3390/ijerph120504921

Figure Lengend Snippet: ( A ) Schematic overview of a microarray slide consisting of two blocks. Each block has four identical grids of probes; ( B ) Glass slide during loading procedure of a hybridization mixture; ( C ) Visualization of an hybridized slide after scanning: the green spots indicate the Cy3-Marker spotted at the four corners of the slide; the red spots indicate the positions where a fluorescent fragment of Amphora coffeaeformis SIT gene recognized the complementary oligonucleotide probes; the green square indicates one of the four sectors of one block.

Article Snippet: Fluorescence scanning of each slide was performed with a microarray scanner (TECAN LS Reloaded TM Microarray Scanner) for the spots corresponding to samples and markers, at 635 nm and at 532 nm, respectively.

Techniques: Microarray, Blocking Assay, Hybridization, Marker

Journal: International Journal of Environmental Research and Public Health

Article Title: Molecular Detection of a Potentially Toxic Diatom Species

doi: 10.3390/ijerph120504921

Figure Lengend Snippet: DNA sequence of the fragments of elongation factor eEF1-a ( A ) and SIT ( B ) obtained by PCR amplification from Amphora coffeaeformis chromosomal DNA. The sense and anti-sense probes specifically designed on this sequence and used for microarray analysis are indicated with black arrows and grey arrows, respectively.

Article Snippet: Fluorescence scanning of each slide was performed with a microarray scanner (TECAN LS Reloaded TM Microarray Scanner) for the spots corresponding to samples and markers, at 635 nm and at 532 nm, respectively.

Techniques: Sequencing, Amplification, Microarray

Journal: International Journal of Environmental Research and Public Health

Article Title: Molecular Detection of a Potentially Toxic Diatom Species

doi: 10.3390/ijerph120504921

Figure Lengend Snippet: Quantification results of microarray hybridization experiments targeting SIT gene of Amphora coffeaeformis . On the Y-axis are reported the probes producing a fluorescent signal after hybridization: yellow bars correspond to probes displaying high specificity, green bars indicate probes with lower specificity, while gray bars correspond to probes producing false-positive signals. The fluorescence intensity signals are reported on the histogram bars.

Article Snippet: Fluorescence scanning of each slide was performed with a microarray scanner (TECAN LS Reloaded TM Microarray Scanner) for the spots corresponding to samples and markers, at 635 nm and at 532 nm, respectively.

Techniques: Microarray, Hybridization, Fluorescence